Pig Breeding 2025 №3(83-84)

THE IMPACT OF HYDROGEN SULFIDE EMISSIONS FROM LIVESTOCK FACILITIES ON THE AIR QUALITY OF THE WORKING AREA OF BUILDINGS AND THE ENVIRONMENT (REVIEW)

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UDC: 636.083:631.95:504.3

DOI: 10.37143/2786-7730-2025-5-6(83-84)7

REFERENCIS АРА style: Nebylytsia, M. S., Boiko, O. V., Havrish, O. M., Honchar, O. F., Voloshchuk, O. V., & Osokina, T. H. (2025). Vplyv emisii sirkovodniu vid obiektiv tvarynnytstva na yakist povitria robochoi zony budivel i dovkillia (ohliadova) [The impact of hydrogen sulfide emissions from livestock facilities on the air quality of the working (review)]. Svynarstvo i Ahropromyslove Vyrobnytstvo [Pig Breeding and Agroindustrial Production]. Poltava, 5–6(83–84), 95–121 [in Ukrainіаn]. 10.37143/2786-7730-2025-5-6(83-84)7

M. S. Nebylytsia, PhD (Agricult.), Head of the Department of Animal Husbandry and Production of Organic Products
ORCID:https://orcid.org/0000-0001-5509-8787
E-mail:nebilitsia@ukr.net
Cherkasy Research Station of Bioresources NAAS, 76, Pasterivska str. , Cherkasy, Ukraine, 18036
O. V. Boiko, PhD (Agricult.), Senior Researcher, Director
ORCID:https://orcid.org/0000-0002-3917-5583
E-mail:bioresurs.ck@ukr.net
Cherkasy Research Station of Bioresources NAAS, 76, Pasterivska str. , Cherkasy, Ukraine, 18036
O. M. Gavrysh, PhD (Agricult.), Senior Researcher, Deputy Director for Research
ORCID:https://orcid.org/0000-0002-8632-6508
E-mail:gavrish.olexandr@gmail.com
Cherkasy Research Station of Bioresources NAAS, 76, Pasterivska str. , Cherkasy, Ukraine, 18036
Honchar O. F. O. F. Honchar, PhD (Agricult.), Senior Research Scientist , Head of the Department of Biodiversity and Ecology
ORCID:https://orcid.org/0000-0032-3269-9767
E-mail:of.gonchar@gmail.com
Cherkasy Research Station of Bioresources NAAS, 76, Pasterivska str. , Cherkasy, Ukraine, 18036
O. V. Voloshchuk, PhD (Agricult.), Deputy Head of the Department of Animal Husbandry and Production of Organic Products
ORCID:https://orcid.org/0009-0007-1999-864Х
E-mail:0970293469@ukr.net
Cherkasy Research Station of Bioresources NAAS, 76, Pasterivska str. , Cherkasy, Ukraine, 18036
Osokina T. G. Researcher, Department of Biodiversity and Ecology
ORCID:https://orcid.org/0009-0006-8840-9493
E-mail:osokina_t@ukr.net
Cherkasy Research Station of Bioresources NAAS, 76, Pasterivska str. , Cherkasy, Ukraine, 18036

Manuscript was received/ 19.05.2025

Received after review/ 03.06.2025

Accepted for printing 28.06.2025

Available online/ 30.12.2025

Abstract

Objective. To identify the main sources of H2S emissions in livestock farming. To quantify its concentration levels in the air of the working area and emission factors into the outside air. To assess the toxicometric effect of H2S on the health of animals and humans at different levels of exposure. To determine ways to minimize environmental pollution. Methods. A desk-based analysis of literary, scientific, and regulatory materials and an evaluation method were used to summarize the data collected. Results. According to literature data, hydrogen sulfide (H2S) is a colorless, flammable gas with the odor of rotten eggs. It is heavier than air, therefore it is most dangerous when it is formed as a result of the decomposition of organic matter in sewage systems, cesspools, and tanks for storing liquidmanure. If precautions are not taken, it can cause poisoning of people working on cleaning and maintaining these facilities. Inefficient storage and disposal systems for manure and poultry droppings can lead to the accumulation of H2S and its emissions into the environment. Hydrogen sulfide exhibits a peculiar behavior of releasing bubbles in tanks with liquid manure. There are data on unexpected outbreaks of H2S release and a sharp increase in its concentration from manure storage tanks. In addition, an increase in H2S concentration occurs in livestock buildings, especially during manure removal operations. This can lead to the accumulation of high concentrations of H2S in the air of the working area. At H2S concentrations above 100 ppm, olfactory receptors completely lose their functions. This makes the smell of gas an ineffective warning of danger The negative consequences of H2S emissions are manifested by toxicological effects on animal and human health. The gas can cause headaches, nausea, dizziness, and at concentrations above 700 ppm – severe poisoning and death. The weighted average concentration level of H2S in the air of the working area of various types of livestock buildings is mainly less than 1000 ppb. The average daily emission factors of hydrogen sulfide from premises for dairy cows range from 401 to 7162 mg/day per cow; from poultry houses - from 462 to 508 mg AU-1 (conditional unit of animals 500 kg); from pig houses - from 220 to 1250 mg AU-1 day-1 . H2S emissions from anaerobic lagoons for storing liquid pig manure are 492.5 mg/m2•day-1 . Conclusions.To reduce hydrogen sulfide emissions, it is necessary to implement effective manure disposal systems, improve ventilation in livestock facilities, and use balanced animal feeding rations.

Keywords: hydrogen sulfide, concentration, emission, livestock building, working area, level of impact, method of minimizing pollution

REFERENCES

1. Natsionalna dopovid pro stan navkolyshnoho pryrodnoho seredovyshcha v Ukraini u 2021 rotsi. [National report on the state of the environment in Ukraine in 2021.] / Ministerstvo zakhystu dovkillia ta pryrodnykh resursiv Ukrainy [in Ukrainian]. Retrieved from https://mepr.gov.ua/wp-content/uploads/2023/01/Natsdopovid-2021-n.pdf (date of access: 25.04.2025).
2. Sirkovoden. Hydrogen Sulfide [in Ukrainian]. Retrieved from https://empendium.com/ua/manual/chapter/B72.XIII.C.14 (date of access: 25.04.2025).
3. USEPA. (2001). Emissions from animal feeding operations, In U.S. Environmental Protection Agency Emission Standards Division Office of Air Quality Planning and Standards Research Triangle Park, NC 27711, August 15, 2001. Р. 32–33. Retrieved from https://www.epa.gov/sites/default/files/2020-10/documents/draftanimalfeed.pdf (date of access: 25.04.2025).
4. Arogo, J., Zhang, R. H., Riskowski, G. L., & Day, D. L. (2000). Laboratory study (Vol. 43). St. Joseph, MI, ETATS-UNIS: American Society of Agricultural Engineers.
5. Ministry of Agrarian Policy of Ukraine (2006). Systemy vydalennia, obrobky, pidhotovky ta vykorystannia hnoiu: VNTP-APK-09.06 [5. Systems for removing, treating, preparing, and using manure] [in Ukrainian]. Retrieved from https://agro.vobu.ua/wp-content/uploads/2021/11/VNTP-APK-09.06.pdf (date of access: 29.04. 2025).
6. Lim, T.-T., Heber, A. J., Ni, J.-Q., Kendall, D., & Richert, B. T. (2004). Effects of manure removal strategies on odor and gas emission from swine finishing. Transactions of the ASAE, 47(6), 2041–2050. https://doi.org/10.13031/2013.17801
7. Hoff, S. J., Bundy, D. S., Nelson, M. A., Zelle, B. C., Jacobson, L. D., Heber, A. J., Ni, J.-Q. ... & Beasley, D.B. (2006). Emissions of ammonia, hydrogen sulfide, and odor before, during and after slurry removal from a deep-pit swine finisher. J. of the Air & Waste Management Association, 56, 581–590. https://doi.org/10.1080/10473289.2006.10464472
8. Aneja, V. P., Blunden, J., Roelle, P. A., Schlesinger, W. H., Knighton, R., Niyogi, D. ...& Duke C. S. (2008). Workshop on agricultural air quality: state of the science. Atmospheric Environment, 42(14), 3195–3208. https://doi.org/10.1016/j.atmosenv.2007.07.043
9. Atia, A., Haugen-Kozyra, K., & Amrani, M. (2004). Ammonia and hydrogen sulfide emissions from livestock production. Alberta: Agriculture, Food and Rural Development, 229–272. Retrieved from https://www1.agric.gov.ab.ca/$department/deptdocs.nsf/all/epw8313/$file/chapter7.pdf (date of access: 25.04.2025).
10. Maasikmets, M., Teinemaa, E., Kaasik, A., & Kimmel, V. (2015). Measurement and analysis of ammonia, hydrogen sulphide and odour emissions from the cattle farming in Estonia. Biosystems Engineering, 139, 48–59. https://doi.org/10.1016/j.biosystemseng.2015.08.002
11. Blanes-Vidal, V., Guàrdia, M., Dai, X. R., & Nadimi, E. S. (2012). NH3, CO2 and H2S emissions during swine wastewater management: characterization of transient emissions after air-liquid interface disturbances. Atmos. Environ., 54, 408–418. https://doi.org/10.1016/j.atmosenv.2012.02.046
12. Van Huffell, K., Hansen, M. J., Feilberg, A., Liu, D., & Van Langenhove, H. (2016). Level and distribution of odorous compounds in exhaust air of pigs from combined room and pit ventilation. Agric. Ecosyst. Environ., 218, 209–219. https://doi.org/10.1016/j.agee.2015.11.020
13. Mostafa, E., Holscher, R., Diekmann, B., Gali, A. E., & Buescher, W. (2017). Evaluation of two methods for reducing indoor air pollution in forced-ventilated pig houses in the fattening area. Atmos. Pollut. Res., 8, 428–438. https://doi.org/10.1016/j.apr.2016.11.003
14. Yeo, W.-H., Lee, I.-B., Kim, R.-W., Lee, S.-Y., & Kim, J.-G. (2019). Computational evaluation of hydrodynamics of pig house ventilation systems to improve the indoor growing environment. Biosystems Engineering, 186, 259–278. https://doi.org/10.1016/j.biosystemseng.2019.08.007
15. Saha, K. K., Zhang, G., Cai, P. & Bjerg, B. (2010). Effects of partial septic tank ventilation system on indoor air quality and ammonia emissions from a pig fattening house. Biosyst. Eng., 105, 279–287. https://doi.org/10.1016/j.biosystemseng.2009.11.
16. Guo L., Zhao B., Jia Y., He F., Chen W. (2022). Strategies for reducing air pollution in mechanically ventilated livestock and poultry houses – a review. Atmosphere., 13(3), 452. https://doi.org/10.3390/atmos13030452
17. Park, J., Seok, J., Lee, S., Kwon, O., Lee, K., Heo, Y., & Yoon, Ch. (2015). Ammonia and Hydrogen Sulfide Monitoring in Broiler Barns and Cattle Barns. J Environ Health Sci., 41(5), 277–288. http://dx.doi.org/10.5668/JEHS.2015.41.5.277
18. Hydrogen Sulfide in Workplace Atmospheres: Method Number ID-141 / Occupational Safety and Health Administration, U.S. Department of Labor, Washington D.C. 2010. URL: https://dnacih.com/niosh/nioshdbs/oshameth/id141/id141.html (date of access: 25.04.2025).
19. OSHA (2005). Hydrogen Sulfide in Workplace Atmospheres: Method Number ID- 141. Occupational Safety and Health Administration, U.S. Department of Labor, Washington D.C. Retrieved from https://www.osha.gov/hydrogen-sulfide/hazards (date of access: 25.04.2025).
20. National Institute for Occupational Safety and Health. (2005). NIOSH Pocket Guide to Chemical Hazards: Hydrogen Sulfide. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Cincinnati, Ohio. September 2005. Retrieved from http://www.cdc.gov/Niosh/npg/npgd0337.html (date of access: 30. 04. 2025).
21. Lim. T.-T., Heber, A. J., & Ni, J.-Q. (2003). Air quality measurements at a laying hen house: odor and hydrogen sulfide emissions. In: International Symposium on Control of Gaseous and Odor Emissions from Animal Production Facilities, Horsens, Denmark. Danish Institute of Agricultural Sciences, Foulum, Denmark, 273–282.
22. Zhu, J., Jacobson, L., Schmidt, D., & Nicolai, R. (2000). Daily variations in odor and gas emissions from animal facilities. Applied Engineering in Agriculture 16, 153–158. https://doi.org/10.13031/2013.5067
23. Kafle, G. K., & Chen, L. (2014). Emissions of Odor, Ammonia, Hydrogen Sulfide, and Volatile Organic Compounds from Shallow-Pit Pig Nursery Rooms. J. of Biosystems Eng., 39(2), 76–86. http://dx.doi.org/10.5307/JBE.2014.39.2.076
24. Guarrasi, J., Trask, C., & Kirychuk, Sh. (2015). A Systematic Review of Occupational Exposure to Hydrogen Sulfide in Livestock Operations. J of Agromedicine, 20(2), 225–236. https://doi.org/10.1080/1059924X.2015.1009667
25. Ni, J. Q., Diehl, C. A., Heber, A. J. еt al. (2013). Emission of pollutants from multi- storey poultry houses with two-year continuous monitoring. Proceedings of the International Symposium on Gas and Dust Emissions from Livestock (EMILI 2012) 10-13 June 2012 in Saint- Malo, France. IFIP – Institut Technique du Porc, 73–77.
26. Ni, J. Q., Heber, A. J., Diehl, C. A., & Lim, T. L. (2000). Ammonia, hygrogen sulphide and crbon dioxide release from pig manure in under-floor deep pits. J. Agric. Eng. Res., 77(1), 53–66. https://doi.org/10.1006/jaer.2000.0561
27. Bashchenko, M. I., Voloshchuk, V. M., Ivanov, V. O. ta in. (2021). Metodyka multy-parametrychnoi otsinky mikroklimatu tvarynnytskykh prymishchen metodom bezperervnoi avtomatychnoi reiestratsii [Methodology of multi-parametric estimation of microclimate of livestock settlements by continuous automatic registration method] / Research Station of Bioresources of the NAAS [in Ukrainian]. Retrieved from https://bioresurs.ck.ua/publications/ (date of access: 30.04.2025).
28. Boiko, O. V., Nebylytsia, M. S., Demydenko, O. V. et al. (2024). Metodyka vyznachennia pokaznykiv emisii parnykovykh haziv ta deiakykh zabrudniuvalnykh rechovyn vid silskohospodarskykh obiektiv i ahrolandshaftiv metodom bezperervnoi avtomatychnoi reiestratsii. [Methodology for determining indicators of greenhouse gas emissions and some pollutants from agricultural facilities and agricultural landscapes by the method of continuous automatic registration] [in Ukrainian]. Retrieved from https://bioresurs.ck.ua/о-бойко-м-небилиця-о- демиденко-о-гаври/ (date of access: 30.11.2025).
29. Nebylytsia, M. S., & Boiko, O. V. (2022). Multyparametrychna otsinka mikroklimatu tvarynnytskykh prymishchen metodom bezperervnoi avtomatychnoi reiestratsii. [Multiparametric assessment of the microclimate of livestock premises using the method of continuous automatic registration]. Svynarstvo [Pig farming]. Poltava, TOV «Firma-«Tekhservis», 77–78, 106–116 [in Ukrainian]. https://doi.org/10.37143/0371-4365-2022-77-78-09
30. Bogan, B. W., & Heber, A. J. (2013). Air pollution emissions from dairy farms: a literature review. Proceedings of the International Symposium on Gas and Dust Emissions from Livestock (EMILI 2012) 10-13 June 2012 in Saint-Malo, France. IFIP – Institut Technique du Porc, 18–21. Retrieved from https://www.rmtelevagesenvironnement.org/backoffice/uploads/Symposium_Emili2012.pdf (date of access: 30.11.2025).
31. Bogan, B. W. et al. (2022). National Air Emissions Monitoring Study: Dairy Industry Data. Freestall Premises and Milking Center in New York State. – NY5B Site. Final Report. Purdue University, Lafayette, IN / Prepared by: U.S. Environmental Protection Agency Office of Air and Radiation Office of Air Quality Planning and Standards 109 T.W. Alexander Drive Research Triangle Park, N.C. 27709., 13–14. Retrieved from https://www.epa.gov/system/files/documents/2024-11/historical-dairy_preliminarydraft_report.pdf (date of access: 30.11.2025).
32. Lim, T. T. et al. (2022). National Air Emission Monitoring Study: Emission Data from Two Freestalls and a Milking Parlor on a Dairy Farm in Indiana State – Site IN5B. Purdue University, Lafayette, IN 2010 / Prepared by: U.S. Environmental Protection Agency Office of Air and Radiation Office of Air Quality Planning and Standards 109 T.W. Alexander Drive Research Triangle Park, N.C. 27709, 12–13. Retrieved from https://www.epa.gov/system/files/documents/2024-11/historical-dairy_preliminarydraft_report.pdf (date of access: 30.11.2025).
33. Cortus et al. (2022). National Air Emissions Monitoring Study: Data from Two Freestall Dairy Facilities in the state of Wisconsin – Site WI5B, Final Report. Purdue University, Lafayette, Vі, 2010 / Prepared by: U.S. Environmental Protection Agency Office of Air and Radiation Office of Air Quality Planning and Standards 109 T.W. Alexander Drive Research Triangle Park, N.C. 27709, 16. Retrieved from https://www.epa.gov/system/files/documents/2024-11/historical-dairy_preliminarydraft_report.pdf (date of access: 30.04.2025).
34. Ramirez J.C. et al. (2022). National Air Emissions Monitoring Study: Data from Two Freestall Dairy Facilities in Washington State WA5B, Final Report. Purdue University, Lafayette, Va, 2010 / Prepared by: U.S. Environmental Protection Agency Office of Air and Radiation Office of Air Quality Planning and Standards 109 T.W. Alexander Drive Research Triangle Park, N.C. 27709, 14–15. Retrieved from https://www.epa.gov/system/files/documents/2024-11/historical-dairy_preliminarydraft_report.pdf (date of access: 30.11.2025).
35. Bottenheim, J. W., & Strauss, O. P. (1980). Gas-phase chemistry of clean air at 55 degrees north latitude. Environ Sci Technol., 14, 709–718. https://doi.org/10.1021/es60166a010
36. Fuller, D. K., & Suruda, A. J. (2000). Occupationally-related hydrogen sulfide deaths in the United States from 1984 to 1994. J OccupEnviron Med., 42, 939–942. Retrieved from https://journals.lww.com/joem/fulltext/2000/09000/occupationally_related_hydrogen_sulfide_dea ths_in.19.aspx (date of access; 25.04.2025).
37. Moretti, M., Ballardini, M., Ciodambro, S., Tronconi, L., Osculati, A., Freni, F., Vignali, S., & Morini, L. (2020). Fatal poisoning of four farm workers: distribution of hydrogen sulfide and thiosulfate in 10 different biological matrices. Forensic Sci International, 316, 110525. https://doi.org/10.1016/j.forsciint.2020.110525
38. Masure, R. (1950). La Keratoconjunctivite des filatures de viscose; etude clinique and experiementale. Rev Belge Pathol., 20, 297–341.
39. Nordstrom, G. A. (1975). A study of the response of calves to ammonia and hydrogen sulfide. Dissertation, University of Alberta, Department of Agricultural Engineering,Edmonton, Alberta. Internet Archive. Retrieved from https://archive.org/details/Nordstrom1975/page/n9/mode/2up (date of access: 25.04.2025).
40. Michal, F. V. (1950). Eye lesions caused by hydrogen sulfide. Cesk Ophthalmol, 6, 5–8.
41. Alberta Ecology Centre (1986). Morphological observations on rats exposed to an atmosphere containing 0.56 or 420 mg/m3 hydrogen sulfide for six hours. AECV86-A1, Alberta. Retrieved from https://archive.org/details/morphologicalobs00albe (date of access: 25.04.2025).
42. Kosmider, S., Rogala, E., & Pacholek, A. (1967). Electrocardiographic and histochemical studies of the heart muscle in acute experimental hydrogen sulfide poisoning. Arch Immunol Ther Exp., 15, 731–740. Retrieved from https://pubmed.ncbi.nlm.nih.gov/4174426/ (date of access: 25.04.2025).
43. Dorman, D. С., Struve, M. F., Gross, E. A., Brenneman, K. A. (2004). Respiratory toxicity of hydrogen sulfide inhalation in Fischer-344, Sprague-Dawley rats, and B6C3F1 mice after subchronic (90-day) exposure. Toxicology and Applied Pharmacology, 198(1), 29–39. https://doi.org/10.1016/j.taap.2004.03.010
44. Elovaara, E., Tossavainen, A., & Savolainen, H. (1978). Effects of subclinical hydrogen sulfide intoxication on mouse brain protein metabolism. Exp Neurol, 62, 93–98. https://doi.org/10.1016/0014-4886(78)90043-2
45. Savolainen, H., Tenhunen, R., Elovaara, E., & Tossavainen, A. (1980). Cumulative biochemical effects of repeated subclinical hydrogen sulfide intoxication in mouse brain. Int Arch Occup Environ Health, 46, 87–92. https://doi.org/10.1007/bf00377463
46. Higuchi, Y., & Fukamachi, M. (1977). Behavioral studies on toxicity of hydrogen sulfide by means of conditioned avoidance responses in rats. Folia Pharmacol Jap, 73, 307–319 [Japanese]. https://doi.org/10.1254/fpj.73.307
47. Prior, M. G., Sharma, A. K., Yong, S., & Lopez, А. (1988). Concentration-time interactions in hydrogen sulphide toxicity in rats. Can J Vet Res., 52, 375–379. Retrieved from https://pmc.ncbi.nlm.nih.gov/articles/PMC1255467/ (date of access: 25.04.2025).
48. Tansy, M. F., Kendall, F. M., Fantasia, J., Landin, W. E., Oberly, R., & Sherman, W. (1981). Acute and subchronic toxicity studies of rats exposed to vapors of methyl mercaptan and other reduced-sulfur compounds. J Toxicol Environ Health, 8, 71–88. https://doi.org/10.1080/15287398109530051
49. Prior, M., Green, F., Lopez, A., Balu, A, DeSanctis, G. T, & Fick, G. (1990). Capsaicin pretreatment modifies hydrogen sulphide-induced pulmonary injury in rats. Toxicol Pathol. 18:279–288. https://doi.org/10.1177/019262339001800206
50. Khan, A. A., Schuler, M. M., Prior, M. G., Yong, S., Coppock, R. W., Florence, L. Z., & Lilly, L. E. (1990). Effects of hydrogen sulfide exposure on lung mitochondrial respiratory chain enzymes in rats. Toxicol Appl Pharmacol, 103, 482–490. https://doi.org/10.1016/0041- 008x(90)90321-k
51. Lopez, A., Prior, M., Yong, S., Albassam, M., & Lillie, L. E (1987). Biochemical and cytological alter ations in the respiratory tract of rats exposed for 4 hours to hydrogen sulfide. Fundam Appl Toxicol, 9, 753–762. https://doi.org/10.1016/0272-0590(87)90182-5
52. Lopez, A., Prior, M., Lillie, L. E., Gulayets С., & Atwa, O. S. (1988a). Histologic and ultrastructural alterations in lungs of rats exposed to sub-lethal concentrations of hydrogen sulfide. Vet Pathol., 25, 376–384. https://doi.org/10.1177/030098588802500507
53. Lopez, A., Prior, M., Yong, S., Lillie, L., & Lefebvre, M. (1988). Nasal lesions in rats exposed to hydrogen sulfide for four hours. Am J Vet Res. 49:1107–1111. Retrieved from https://pubmed.ncbi.nlm.nih.gov/3421534/ (date of access: 25.04.2025).
54. Beck, J. F., Cormier, F., & Donini, J. C. (1979). Combined toxicity of ethanol and hydrogen sulfide. Toxicol Lett., 3, 311–313. https://doi.org/10.1016/0378-4274(79)90009-2
55. Kage, S., Nagata, T., Kimura, K., Kudo, K., & Imamura, T. (1992). Usefulness of thiosulfate as an indicator of hydrogen sulfide poisoning in forensic toxicological examination: A study with animal experiments. Jpn J Forensic Toxicol., 10(3), 223–227.
56. Smith, R. P., & Gosselin, R. E. (1964). The influence of methemoglobinemia on the lethality of some toxic anions: II. Sulfide. Toxicol Appl Pharmacol, 6, 584–592. https://doi.org/10.1016/0041-008x(64)90090-0
57. Lopez, A., Prior, M. G., Reiffenstein, R. J., & Goodwin, L. R. (1989). Peracute toxic effects of inhaled hydrogen sulfide and injected sodium hydrosulfide on the lungs of rats. Fundam Appl Toxicol., 12, 367–373. https://doi.org/10.1016/0272-0590(89)90053-5
58. AfTSaDR U.S. Department of Health and Human Services, Division of Toxicology and Environmental Medicine/Division of Applied Toxicology (ed.) (2016). Toxicological Profile of Hydrogen Sulfide and Carbonyl Sulfide. Atlanta, GA. Retrieved from https://www.ncbi.nlm.nih.gov/books/NBK591605/ (date of access: 25.04.2025).
59. Ministry of Agrarian Policy of Ukraine (2005). Svynarski pidpryiemstva (kompleksy, fermy, mali fermy): VNTP-APK-01.05 [Departmental norms of technological design. Pig enterprises (complexes, farms, small farms)] [in Ukrainian]. Retrieved from https://lugdpss.gov.ua/images/bezpechnist_veterynariya/Svynarski-pidpryyemstva-VNTP-APK- 02.05.pdf (date of access: 25.04.2025).
60. Ministry of Agrarian Policy of Ukraine (2005). Skotarski pidpryiemstva (kompleksy, fermy, malifermy): VNTP-APK-02.05 [Departmental norms of technological design. Cattle enterprises (complexes, farms, small farms)] [in Ukrainian]. Retrieved from https://dbn.co.ua/load/normativy/vntp/14-1-0-1039 (date of access: 25.04.2025).
61. Ministry of Agrarian Policy of Ukraine (2007). Pidpryiemstva zvirivnytstva ta krolivnytstva: VNTP-APK-05.07 [Departmental norms of technological design. Animal and rabbit breeding enterprises] [in Ukrainian]. Retrieved from https://online.budstandart.com/ua/catalog/doc-page.html?id_doc=67808 (date of access: 25.04.2025).
62. Schiffman, S. S., Auvermann, B. W., & Bottcher, R. W. (2002). Health effects of aerial emissions from animal production waste management systems. National Center for Manure and Animal Waste Management White Papers (North Carolina State University, Raleigh, NC. Retrieved from https://elibrary.asabe.org/azdez.asp? JID=1&AID=23886&CID=aqwm2007&T=1 (date of access: 25.04.2025).
63. Saeedi, A., Najibi, A., & Mohammadi-Bardbori, A. (2015). Effects of long-term exposure to hydrogen sulfide on human erythrocytes. Intern J of Occupation and Environmental Health, 6(1), 20–25. Reetrieved from https://pubmed.ncbi.nlm.nih.gov/25588222/ (date of access: 25.04.2025).
64. Guidotti Tee L. (2010). Hydrogen Sulfide: Advances in Understanding Human Toxicity. Intern J of Toxicology, 29(6), 569–581. https://doi.org/10.1177/1091581810384882
65. Helmi, N., Pripp-Boos, K., Vons, K., Lenoir, V., Abou-Hamdan, A., Guedouari- Bounihi, H., Lombès, A., & Bouillaud, F. (2014). Oxidation of hydrogen sulfide by human liver mitochondria. Nitric Oxide, 41, 105–112. https://doi.org/10.1016/j.niox.2014.05.011
66. Aneja, V. P., Blunden, J., Roelle, P. A., Schlesinger, W. H., Knighton, R., & Niyogi, D. (2008). Workshop on agricultural air quality: state of the science. Atmospheric Environment, 42(14), 3195–3208. https://doi.org/10.1016/j.atmosenv.2007.07.043
67. Kilburn, K. H. (2012). Human impairment from living near confined animal (hog) feeding operations. J Environ Public Health, 2012, 565690. Retrieved from https://pmc.ncbi.nlm.nih.gov/articles/ PMC3306954/ (date of access: 25.04.2025).
68. U.S. Environmental Protection Agency (1993). Report to Congress on Hydrogen Sulfide Air Emissions Associated with Oil and Natural Gas Production. Research Triangle Park, North Carolina: U.S. Environmental Protection Agency, Office of Air Quality Planning and Standards. EPA-453/R-93-045. Retrieved from https://www.osti.gov/biblio/5394599 (date of access: 25.04.2025).
69. WHO (1981). Environmental health criteria: Hydrogensulfide. Geneva, Switzerland: WorldHealthOrganization. https://www.inchem.org/documents/ehc/ehc/ehc019.htm (date of access: 25.04.2025).
70. Hill, F. B. (1973). Atmospheric sulfur and its links to the biota. Brookhaven Symp Biol., 30, 159–181.
71. Cox, R., & Sheppard, D. (1980). Reactions of OH radicals with gaseous sulfur compounds. Nature. 284, 330–331. https://doi.org/10.1038/284330a0
72. Millero, F. J., Hubinger, S., Fernandez, M., & Garnett, S (1987). Oxidation of H2S in sea water as a function of temperature, pH and ionic strength. Environ Sci Technol, 21, 439– 443. https://doi.org/10.1021/es00159a003
73. Millero, F. J., LeFerriere, A., Fernandez, M., Hubinger, S., & Hershey, J. P. (1989). Oxidationofhydrogensulfidewith H2O2innaturalwaters. EnvironSciTechnol, 23(2), 209–213. https://doi.org/10.1021/es00179a012
74. O’Neil, M. J., Heckelman, P. E., Koch, Ch. B., & Roman, K. J. (eds.) (2001). Hydrogen sulfide. The Merck index. An encyclopedia of chemicals, drugs, and biologicals. Whitehouse Station, NJ: Merck & Co., Inc.
75. Torrance, E. L., & Clemens, G. P. (1982). Physiological and biochemical effects of acute exposure to hydrogen sulfide in fish. Comp Biochem Physiol 1, 71(2), 183–190. https://doi.org/10.1016/0306-4492(82)90034-X
76. Bagarinao, T, & Vetter, R. D. (1992). Sulfide-hemoglobin interactions in the sulfide-tolerant salt marsh California killifish Fundulus parvipinnis. J of Comparative Physiology B, 162, 614–624. https://doi.org/10.1007/BF00296642
77. Fryer, J. M. (2018). Soil Properties That Influence the Occurrence of Hydrogen Sulfide Toxicity in Rice Fields. Graduate Theses and Dissertations Retrieved from https://scholarworks.uark.edu/etd/2640 (date of access: 24.04.2025).
78. Li, P., Li, M., Liu, Zh., Zhao, Y., Qian, X., & Huang, P. (2022). Effect of high temperature and sulfur vapor on the flammability limit of hydrogen sulfide. J of Cleaner Production, 337, 130579. https://doi.org/10.1016/j.jclepro.2022.130579
79. Chen, B., Kozel, J. A., Li, M., O'Brien, S. K., Li, P., & Brown, R. K. (2021). Reduction of acute hydrogen sulfide and ammonia emissions from pig manure during three-hour mixing using granular biochar. Atmosphere, 12(7), 825. https://doi.org/10.3390/atmos12070825
80. Wi, J., Lee, Sh., Kim, A., Kim, A., Lee, M., Kozel, J. A., & Ahn, H. (2019). Evaluation of the effectiveness of a semi-continuous manure pit replenishment system in reducing ammonia and hydrogen sulfide emissions from a pig fattening barn. Atmosphere, 10(4), 170. https://doi.org/10.3390/atmos10040170
81. Vorobel, M. I., Kaplinskyi, V. V., Klym, O. Ya., Dmytrotsa, A. I., & Telushko, H. Ya. (2022). Efektyvnist vplyvu riznykh doz biopreparatu Kapeliukhiv Yarok na riven vydilennia shkidlyvykh haziv z kuriachoho poslidu [Effectiveness of the effect of different doses of the biological preparation Kapelyukhiv Yarok on the level of emission of harmful gases from chicken droppings]. Visnyk ahrarnoi nauky [Bulletin of agricultural science], 2, 67–73 [in Ukrainian].
82. Lim, T. T., Heber, A. J., Ni, J. Q., Sutton, A. L., & Shao, P. (2003). Atmospheric pollutants and trace gases: Odor and gas release from anaerobic treatment lagoons for swine manure. J Environ Qual., 32(2), 406–416. https://doi.org/10.2134/jeq2003.4060
83. Zahn, J. A., Hatfield, J. L., Laird, D. A., Hart, T. T., Do, Y. S., & DiSpirito, A. A. (2001). Functional classification of swine manure management systems based on effluent and gas emission characteristics. Journal of Environmental Quality, 30(2), 635–647. https://doi.org/10.2134/jeq2001.302635x
84. Biletskyi, V. S., & Orlovskyi, V. M. Biohaz [Biogas]. Velyka ukrainska entsyklopediia [Great Ukrainian Encyclopedia] [in Ukrainian]. Retrieved from https://vue.gov.ua/Біогаз (date of access: 25.04.2025)..
85. Sanzhara. R. A., & Lesnovska, O. V. (2024). Tekhnolohiia pererobky vidkhodiv silskohospodarskoho vyrobnytstva [Technology of processing agricultural waste]. Dnipro [in Ukrainian]. Retrieved from https://dspace.dsau.dp.ua/handle/123456789/11992 (date of access: 25.04.2025).
86. Holub, H. A., Dubrovin, V. O., Polishchuk, V. M., Siera, K. M., Marus, A. O., Drahniev, S. V., Sydorchuk, O. V. ...& Shcherbak, S. D. (2015). Biohaz. [Biogas]. Kyiv [in Ukrainian]. Retrieved from http://ir.polissiauniver.edu.ua/bitstream/123456789/5144/1/Biogaz_TM7.pdf (date of access: 29.04.2025). of access: 29.04.2025).
87. Bioenerhetyka v Ukraini [Bioenergy in Ukraine]. Official website UABIO [in Ukrainian]. Retrieved from https://uabio.org/bioenergy-in-ukraine/ (date of access: 22.09.2025)..
88. Biogas desulfurization. Website Knowledge Ridge. Retrieved from https://www.knowledgeridge.com/expert-views/biogas-desulfurization [in Ukrainian] (date of access: 09.06.2024).
89. Dumont, E. (2015). H2S removal from biogas using bioreactors: a review. Intern J of Energy and Environment (IJEE), 6(5), 479–498. Retrieved from http://www.ijee.ieefoundation.org/ (date of access: 22.04.2025).
90. Chemical Products Industries Inc. Hydrogen sulfide removal from natural gas and biogas. Site Chemical Products Industries, Inc. Retrieved from https://www.chemicalproductsokc.com/sulfurtrap-h2s-removal-gas/ (date of access: 30.09.2025).
91. Yadav, A., & Kale, S. (2021). Hydrogen Sulfide Control via Biotrickling Filters. Clean Technologiesand Environmental Policy, 23(3), 457–468. https://doi.org/10.1007/s10098-020-01972-3
92. Guo, L., Zhao, B., Jia, Y., He, F., & Chen, W. (2022). Strategies for reducing air pollution in mechanically ventilated livestock and poultry houses – a review. Atmosphere, 13(3), 452. https://doi.org/10.3390/atmos13030452
93. Ni, J.-Q. (2021). Factors affecting toxic hydrogen sulfide concentrations on swine farms - Sulfur source, release mechanism, and ventilation. J Clean. Prod., 322, 129–126. Retrieved from https://www.sciencedirect.com/science/article/pii/S0959652621033151 (date of access: 22.04.2025).
94. Kafle, G. K., Chen, L., Naibling, H., & He, B. B. (2015). Field evaluation of downflow wood bark-based biofilters for reducing odor, ammonia, and hydrogen sulfide emissions from closed pig houses. J Environ. Manag., 147, 164–174. https://doi.org/10.1016/j.jenvman.2014.09.004
95. Dumont, E., Cabral, F. D. S., LeCloirec, P., & Andrés, Y. (2013). Biofiltration using peat and nutrient synthetic packing material: influence of packing configuration on H2S removal. Environ. Technol, 34, 1123–1129. https://doi.org/10.1080/09593330.2012.736691
96. Nour, M. M., Cheng, Y.-H., Ni, J.-Q., Sheldon, E., Field, W. E. (2021). Summary of injuries 139 and fatalities involving livestock manure storage, handling, and transport operation sin seven 140 central states: 1976–2019. J Agric. Saf. Health, 27, 105–122. http://doi.org/10.13031/jash. 14343
97. Oesterhelweg, L., & Püschel, K. (2008). Death may come on like a stroke of lightning. Phenomenological and morphological aspects of fatalities caused by manure gas. Int. J. Legal. Med. 122(2), 101–107. http://doi.org/10.1007/s00414-007-0172-8
98. Liu, Sh., Ni, J.-K., Radcliffe, J. S., & Vonderohe, C (2017). Hydrogen sulfide emissions from a pig farm affected by crude protein in the diet. J of Environmental Management, 204(1), 136–143. https://doi.org/10.1016/j.jenvman.2017.08.0