GENETICS IN PIG BREEDING: A REVIEW OF UKRAINIAN RESEARCH IN INTERNATIONAL SCIENTIFIC PUBLICATIONS


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UDC: *UDC 575.22:636.082:636.4(477)

DOI: 10.37143/2786-7730-2026-7(85)4

REFERENCIS АРА style:For citation (APA Style): Peka, M. Yu. (2026). Genetics in Pig Breeding: a Review of Ukrainian Research in International Scientific Publications. Svynarstvo i Ahropromyslove Vyrobnytstvo [Pig Breeding and Agroindustrial Production]. Poltava, 7(85), 42–57 [in Ukrainіаn]. https://doi.org/10.37143/2786-7730-2026-7(85)4

M. Y. PekaPhD Student in Biology, Department of Molecular Biology and Biotechnology, School of Biology, V. N. Karazin Kharkiv National University
ORCID:https://orcid.org/0000-0003-0612-1164
E-mail:pekapoltava@gmail.com
V. N. Karazin Kharkiv National University 4 Svobody Sq, Kharkiv, Ukraine, 61022ROR: https://ror.org/03ftejk10

Manuscript was received/ 16.04.2026

Received after review/ 28.04.2026

Accepted for printing/ 22.06.2026

Available online/ 30.06.2026

Abstract

Objective.The aim of this study was to systematically analyze publications on pig genetics authored by Ukrainian researchers and indexed in the Scopus database, with emphasis on identifying the main research directions, methodological approaches, frequently studied genetic polymorphisms, and pig breeds. An additional objective was to assess how Ukrainian research in pig breeding genetics is represented within the international scientific space. Methods. A systematic literature search was conducted in the Scopus database. The selection process included identification, screening of titles and abstracts, and eligibility assessment based on full-text analysis. Inclusion criteria comprised original research articles and reviews focused on pig genetics with at least one Ukrainian-affiliated author. Exclusion criteria included conference abstracts, studies not directly related to pig genetics and those violating ethical standards. Data analysis focused on genetic markers, breeds, traits studied, and methodological approaches. Results. The initial search yielded 126 records, of which 39 publications met the inclusion criteria after screening and eligibility assessment. The dataset demonstrated thematic heterogeneity, with 29 studies directly addressing molecular genetic variability, predominantly through analysis of single nucleotide polymorphisms. The most frequently studied gene was MC4R, followed by RYR1, ESR1, PRLR, LEP, and LEPR. Research was primarily conducted on Ukrainian Large White pigs, with limited representation of other breeds. The main areas of investigation included growth traits, reproductive performance, and meat quality characteristics. Methodologically, the majority of studies employed PCR-based approaches, especially PCR- RFLP, while genome-wide approaches such as sequencing and GWAS were absent. The analysis also indicated a relatively small number of Scopus-indexed publications compared to the broader range of national research. Conclusions. Ukrainian research in pig genetics, as represented in internationally indexed literature, is characterized by a focus on candidate and causal gene polymorphisms and marker-assisted selection. However, its international visibility remains limited, and methodological approaches lag behind current global trends. Future development requires both the adoption of genome-wide technologies and increased integration into international scientific publishing to enhance the accessibility and impact of Ukrainian research.

Key words: pig genetics, pig breeding, Ukraine, polymorphism, SNP, marker-assisted selection, candidate genes, Scopus, PCR-RFLP.

REFERENCES

  • 1. Margeta, V., Škorput, D., Djurkin Kušec, I., Kralik, Z., Kušec, G., & Gvozdanović, K. (2025). A Comprehensive Review: Molecular and Genealogical Methods for Preserving the Genetic Diversity of Pigs. Applied Sci, 15(6), 3394. https://doi.org/10.3390/app15063394
  • 2. Panigrahi, M., Rajawat, D., Nayak, S. S., Bose, A., Bharia, N., Singh, S., Sharma, A., & Dutt, T. (2025). Advancements in Animal Breeding: From Mendelian Genetics to Machine Learning. Intern J of molecular Sci, 26(23), 11352. https://doi.org/10.3390/ijms262311352
  • 3. Krupa, E., Moravčíková, N., Krupová, Z., & Žáková, E. (2021). Assessment of the Genetic Diversity of a Local Pig Breed Using Pedigree and SNP Data. Genes, 12(12), 1972. https://doi.org/10.3390/genes12121972
  • 4. Samorè, A. B., Buttazzoni, L., Gallo, M., Russo, V., & Fontanesi, L. (2015). Genomic selection in a pig population including information from slaughtered full sibs of boars within a sib-testing program. Animal : an Intern J of Animal Biosci, 9(5), 750–759. https://doi.org/10.1017/S1751731114002924
  • 5. Samorè, A. B., & Fontanesi, L. (2016). Genomic selection in pigs: state of the art and perspectives. Italian J of Animal Sci, 15(2), 211–232. https://doi.org/10.1080/1828051X.2016.1172034
  • 6. Su, R., Lv, J., Xue, Y., Jiang, S., Zhou, L., Jiang, L., Tan, J., Shen, Z., Zhong, P., & Liu, J. (2025). Genomic selection in pig breeding: comparative analysis of machine learning algorithms. Genetics, Selection, Evolution : GSE, 57(1), 13. https://doi.org/10.1186/s12711-025-00957-3
  • 7. Biscarini, F., Nicolazzi, E. L., Stella, A., Boettcher, P. J., & Gandini, G. (2015). Challenges and opportunities in genetic improvement of local livestock breeds. Frontiers in Genetics, 6, 33. https://doi.org/10.3389/fgene.2015.00033
  • 8. Misztal, I., Aguilar, I., Lourenco, D., Ma, L., Steibel, J. P., & Toro, M. (2021). Emerging issues in genomic selection. J of Animal Sci, 99(6), skab092. https://doi.org/10.1093/jas/skab092
  • 9. Voitenko, S. L. (2024). Pigs of meat breeds in Ukraine and the need for the revival of pig breeding. Animal Breeding and Genetics, 67, 29-45. https://doi.org/10.31073/abg.67.04
  • 10. Balatsky, V. N., Saienko, A. M., Pena, R. N., Buslyk, T. V., & Gibolenko, O. S. (2015). Genetic diversity of pig breeds on ten production quantitative traits loci. Cytology and Genetics, 49(5), 299–307. https://doi.org/10.3103/S0095452715050023
  • 11. Balatsky, V., Bankovska, I., Pena, R. N., Saienko, A., Buslyk, T., Korinnyi, S., & Doran, O. (2016). Polymorphisms of the porcine cathepsins, growth hormone-releasing hormone and leptin receptor genes and their association with meat quality traits in Ukrainian Large White breed. Molecular Biology Reports, 43(6), 517–526. https://doi.org/10.1007/s11033- 016-3977-z
  • 12. Balatsky, V. N., Oliinychenko, Y. K., Buslyk, T. V., Bankovska, I. B., Korinnyi, S. N., Saienko, A. M., & Pochernyaev, K. F. (2021). Associations of QTL Region Genes of Chromosome 2 with Meat Quality Traits and Productivity of the Ukrainian Large White Pig Breed. Cytology and Genetics, 55(1), 53–62. https://doi.org/10.3103/S0095452721010023
  • 13. Calderon Martinez, E., Ghattas Hasbun, P. E., Salolin Vargas, V. P., García- González, O. Y., Fermin Madera, M. D., Rueda Capistrán, D. E., Campos Carmona, T., Sanchez Cruz, C., & Teran Hooper, C. (2025). A comprehensive guide to conduct a systematic review and meta-analysis in medical research. Medicine, 104(33), e41868. https://doi.org/10.1097/MD.0000000000041868
  • 14. Brignardello-Petersen, R., Santesso, N., & Guyatt, G. H. (2025). Systematic reviews of the literature: an introduction to current methods. American J of Epidemiology, 194(2), 536–542. https://doi.org/10.1093/aje/kwae232
  • 15. Randles, R., & Finnegan, A. (2023). Guidelines for writing a systematic review. Nurse Education Today, 125, 105803. https://doi.org/10.1016/j.nedt.2023.105803
  • 16. Ibatullin, M., Varchenko, O., Svynous I., Khakhula, B., & Dragan, O. (2019). Organizational and economic bases of pig breeding in Ukraine. Intern J of Management and Business Research, 9(1), 59–72.
  • 17. Kramarenko, A. S., Karatieieva, O. I., Lіuta, I. M., & Kramarenko, S. S. (2024). Genetic and non-genetic factors influencing piglet stillbirth risk. Regulatory Mechanisms in Biosystems, 15(4), 875–881. https://doi.org/10.15421/0224126
  • 18. Voloshynov, V., Povod, M., Mykhalko, O., Verbelchuk, T., Verbelchuk, S., Koberniuk, V., Lavryniuk, O., & Shcherbatiuk, N. (2024). The efficiency of pigs from different genetic origins under industrial conditions in Ukraine. Online J of Animal and Feed Research, 14(4), 225–233. https://doi.org/10.51227/ojafr.2024.27
  • 19. Ostapovets, L., & Starodub, L. (2016). Cytogenetical and biotechnological methods for assessing boar reproductive potential. Bulgarian J of Agricultural Sci, 22, 123–126.
  • 20. Balatskiĭ, V. N., & Lisovskiĭ, I. L. (1998). The genetic polymorphism of somatotropin. TSitologiia i Genetika, 32(2), 92–104.
  • 21. Burkina, V., Rasmussen, M. K., Oliinychenko, Y., & Zamaratskaia, G. (2019). Porcine cytochrome 2A19 and 2E1. Basic and Clinical Pharmacology and Toxicology, 124(1), 32–39. https://doi.org/10.1111/bcpt.13121
  • 22. Guo, L., Miao, Z., Ma, H., & Melnychuk, S. (2020). Effects of maternal vitamin D 3 during pregnancy on FASN and LIPE mRNA expression in offspring pigs. J of Agricultural Sci, 158(1–2), 128–135. https://doi.org/10.1017/S0021859620000210
  • 23. Guo, L., Miao, Z., Ma, H., & Melnychuk, S. (2020). Effects of maternal vitamin D3 concentration during pregnancy on adipogenic genes expression and serum biochemical index in offspring piglets. J of Animal and Feed Sci, 29(2), 125–131. https://doi.org/10.22358/jafs/124041/2020
  • 24. Peka, M., & Balatsky, V. (2024). Bioinformatic approach to identifying causative missense polymorphisms in animal genomes. BMC Genomics, 25(1). https://doi.org/10.1186/s12864-024-11126-z
  • 25. Peka, M., Balatsky, V., Saienko, A., & Tsereniuk, O. (2023). Bioinformatic analysis of the effect of SNPs in the pig TERT gene on the structural and functional characteristics of the enzyme to develop new genetic markers of productivity traits. BMC Genomics, 24(1). https://doi.org/10.1186/s12864-023-09592-y
  • 26. Pochernayev, K. K., Kiynitsa, K. I., Telegeev, G. D., Dybkov, M. V., & Malyuta, S. S. (1996). M13 phage DNA probe informativity rising at studying pig genome. Biopolymers and Cell, 12(3), 64–66. https://doi.org/10.7124/bc.00042F
  • 27. Kubejko, J., Clop, A., Balatsky, V., Pochernyaev, K., Eghbalsaied, S., & Amills, M. (2017). Mitochondrial DNA variation in Ukrainian wild boars. Animal Genetics, 48(6), 725–726. https://doi.org/10.1111/age.12592
  • 28. Balatskiĭ V. N. (1992). Multiple forms of pig somatotropin. TSitologiia i Genetika, 26(5), 32–36.
  • 29. Balatsky, V. N., Saenko, A. M., & Grishina, L. P. (2012). Polymorphism of the estrogen receptor 1 locus in populations of pigs of different genotypes and its association with reproductive traits of Large White sows. Cytology and Genetics, 46(4), 233–237. https://doi.org/10.3103/S0095452712040020
  • 30. Matiiuk, V. V., Saienko, A. M., Vashchenko, P. A., Slynko, V. H., Fesenko, O. G., Peka, M. Y., & Tsereniuk, O. M. (2025). Association of polymorphisms in estrogen and prolactin receptor genes with reproductive traits in sows of rare breeds. Regulatory Mechanisms in Biosystems, 33(1), e25033. https://doi.org/10.15421/0225033
  • 31. Syrovnev, G. I. (2014). Genetic polymorphism of FUT1 and MUC4 loci in a local population of Ukrainian meat bread pigs. Cytology and Genetics, 48(5), 318–322. https://doi.org/10.3103/S0095452714050119
  • 32. Sukhno, V. V., Vashchenko, P. A., Saenko, A. M., Zhukorskyi, O. M., Tserenyuk, O. M., & Kryhina, N. V. (2022). Association of Fut1 and Slc11a1 gene polymorphisms with productivity traits of Large White pigs. Regulatory Mechanisms in Biosystems, 13(3), 225–230. https://doi.org/10.15421/022229
  • 33. Balatskiĭ, V. N., & Pocherniaev, K. F. (1995). The polymorphic BsuRI restriction site of the swine growth hormone gene. TSitologiia i Genetika, 29(1), 45–48. 34. Balatsky, V., Oliinychenko, Y., Sarantseva, N., Getya, A., Saienko, A., Vovk, V., & Doran, O. (2018). Association of single nucleotide polymorphisms in leptin (LEP) and leptin receptor (LEPR) genes with backfat thickness and daily weight gain in Ukrainian Large White pigs. Livestock Sci, 217, 157–161. https://doi.org/10.1016/j.livsci.2018.09.015
  • 35. Balatsky, V. N., Oliinychenko, Y. K., Saienko, A. M., Buslyk, T. V., Bankovska, I. B., Peka, M. Yu., & Doran, O. (2022). Associations of Polymorphisms in Leptin and Leptin Receptor Genes with Meat Quality in Pigs of the Ukrainian Large White Breed. Cytology and Genetics, 56(6), 513–525. https://doi.org/10.3103/S0095452722060020
  • 36. Bordun, O., Khalak, V., Skliarenko, Y., Gutyj, B., Stadnytska, O., Ilchenko, M., Shevchenko, O., Kibenko, N., & Kravchenko, N. (2025). Evaluation of the Genotypes of Pigs of Different Origins According to the RYR1 and LEP Genes. Acta Fytotechnica et Zootechnica, 28(1), 1–6. https://doi.org/10.15414/afz.2025.28.01.1-6
  • 37. Lyadskiy, I. K., Getya, A. A., & Pochernyaev, K. F. (2011). Association of the Asp298Asn polymorphism in the mc4r gene with back fat thickness in pigs of the Large White breed. Cytology and Genetics, 45(2), 106–109.
  • 38. Kozyr, V., Khalak, V., & Povod, M. (2019). DNA-type results swine for MS4R- gene and its association with productivity. AgroLife Scientific J, 8(1), 128–133. 39. Khalak, V. (2020). Fattening and meat qualities of store pigs of large white breed of different intra-breed differentiation by melanocortin-4 receptor gene (MC4R). Scientific Horizons, 23(9), 30–37. https://doi.org/10.48077/scihor.23(9).2020.30-37
  • 40. Khalak, V., Horchanok, A., Kuzmenko, O., Lytvyschenko, L., Prysiazhniuk, N., & Bordun, A. (2021). Interior profile of young pigs of different genotypes and the use of its components for early prediction of quantitative characters. AgroLife Scientific J, 10(2), 92–98. https://doi.org/10.17930/AGL2021211
  • 41. Khalak, V., Voloshchuk, V., Gutyj, B., Zasucha, L., Onyshchenko, A., Ilchenko, M., Ofilenko, N., Pokhyl, V., Pundyk, V., Bezaltychna, O., & Stadnytska, О. (2023). Young pig fattening and meat quality due to varying formation intensities in early ontogenesis and two genotypes of the melanocortin receptor 4 (Mc4r) gene. Veterinarska Stanica, 54(6), 613–624. https://doi.org/10.46419/vs.54.6.10
  • 42. Zhukorskyi, O. M., Tsereniuk, O. M., Sukhno, T. V., Saienko, A. M., Polishchuk, A. A., Chereuta, Y. V., Shaferivskyi, B. S., & Vashchenko, P. A. (2023). The influence of genotype and feeding level of gilts on their further reproductive performance. Regulatory Mechanisms in Biosystems, 14(2), 312–318. https://doi.org/10.15421/022346
  • 43. Tsereniuk, O. M., Vashchenko, P. A., Khokhlov, A. M., Tsybenko, V. H., Shostia, G. M., Saenko, A. M., Peka, M. Y., & Zhukorskyi, O. M. (2023). Comparative characteristics of polymorphisms of melanocortin 4 and ryanodine 1 receptor genes of Myrhorod pigs before and after the African swine fever outbreak. Regulatory Mechanisms in Biosystems, 14(4), 601–608. https://doi.org/10.15421/022387
  • 44. Vashchenko, P. A., Zhukorskyi, O. M., Saenko, A. M., Khokhlov, A. M., Usenko, S. O., Kryhina, N. V., Sukhno, T. V., & Tsereniuk, O. M. (2023). The influence of feeding level on the growth of pigs depending on their genotype. Regulatory Mechanisms in Biosystems, 14(1), 112–117. https://doi.org/10.15421/022317
  • 45. Khalak, V., Bordun, O., Skliarenko, Y., Gutyj, B., Zasukha, L., Horchanok, A., Kuzmenko, O., & Prisjazhnjuk, N. (2025). Study of Polymorphism of the MC4R Locus in Pigs of the Large White Breed. Acta Fytotechnica et Zootechnica, 28(4), 282–287. https://doi.org/10.15414/afz.2025.28.04.282-287
  • 46. Balatskiĭ, V. N., & Metlitskaia, E. N. (2001). DNA diagnosis of porcine stress syndrome and RYRI genotype association with viability of young pigs. TSitologiia i genetika, 35(3), 43–49.
  • 47. Tsereniuk, O. M., Bobrytska, O. M., Miroshnikova, O. S., & Danchuk, O. V. (2020). DNA-type results of Landrace sows for RYR1-gene and its association with productivity. Regulatory Mechanisms in Biosystems, 11(3), 431–437. https://doi.org/10.15421/022066
  • 48. Zhukorskyi, O. M., Tsereniuk, O. M., Vashchenko, P. A., Khokhlov, A. M., Chereuta, Y. V., Akimov, O. V., & Kryhina, N. V. (2022). The effect of the ryanodine receptor gene on the reproductive traits of Welsh sows. Regulatory Mechanisms in Biosystems, 13(4), 367–372. https://doi.org/10.15421/022248
  • 49. Solé, E., González-Prendes, R., Oliinychenko, Y., Tor, M., Ros-Freixedes, R., Estany, J., & Pena, R. N. (2022). Transcriptome shifts triggered by vitamin A and SCD genotype interaction in Duroc pigs. BMC Genomics, 23(1). https://doi.org/10.1186/s12864-021-08244-3
  • 50. Vashchenko, P., Saienko, A., Sukhno, V., Tsereniuk, O., Babicz, M., Shkavro, N., Smołucha, G., & Łuszczewska-Sierakowska, I. (2022). Association of NRAMP1 gene polymorphism with the productive traits of the Ukrainian Large White pig. Medycyna Weterynaryjna, 78(9). https://doi.org/10.21521/mw.6698
  • 51. Saienko, A., Peka, M., Tsereniuk, O., Babicz, M., Kropiwiec-Domańska, K., Onyshchenko, A., Vashchenko, P., & Balatsky, V. (2023). Analysis of polymorphism and development of a molecular-genetic system for genotyping by the telomerase reverse transcriptase (TERT) gene. Biosystems Diversity, 31(4), 436–443. https://doi.org/10.15421/012352
  • 52. Hashim, H. O. & Al-Shuhaib, M. B. (2019). Exploring the Potential and Limitations of PCR-RFLP and PCR-SSCP for SNP Detection: A Review. Journal of Applied Biotechnology Reports, 6, 137-144. https://doi.org/10.29252/JABR.06.04.02
  • 53. Leite, I. G. C., Benard, G., Cavalcanti, S. C., Bollela, V. R., Del Negro, G. M. B., Martinez, R., Gimenes, V. M. F., & Cocio, T. A. (2024). Comparison between PCR-RFLP and sequencing techniques in the analysis of Paracoccidioides spp. biodiversity: limitations and insights into species and variant differentiation. Mycopathologia, 189(6), 97. https://doi.org/10.1007/s11046-024-00902-w
  • 54. Mekonnen, K. T., Lee, D. H., Beyenssa, B. C., Son, A. Y., Cho, Y. G., & Seo, K. S. (2025). Genome-wide association study identifies genetic variants associated with single- parity reproductive loss in three commercial pig breeds. BMC Genomics, 26(1), 1054. https://doi.org/10.1186/s12864-025-12255-9
  • 55. Panda, S., Kumar, A., Gaur, G. K., Ahmad, S. F., Chauhan, A., Mehrotra, A., & Dutt, T. (2023). Genome wide copy number variations using Porcine 60K SNP Beadchip in Landlly pigs. Animal Biotechnology, 34(6), 1891–1899. https://doi.org/10.1080/10495398.2022.2056047
  • 56. Hong, Y., He, X., Wu, D., Ye, J., Zhang, Y., Wu, Z., & Tan, C. (2025). Genome Selection and Genome-Wide Association Analyses for Litter Size Traits in Large White Pigs. Animals : an open access journal from MDPI, 15(12), 1724. https://doi.org/10.3390/ani15121724
  • 57. Wang, X., Wang, L., Shi, L., Zhang, P., Li, Y., Li, M., Tian, J., Wang, L., & Zhao, F. (2022). GWAS of Reproductive Traits in Large White Pigs on Chip and Imputed Whole- Genome Sequencing Data. Intern J of Molecular Sci, 23(21), 13338. https://doi.org/10.3390/ijms232113338
  • 58. Du, H., Zhou, L., Liu, Z., Zhuo, Y., Zhang, M., Huang, Q., Lu, S., Xing, K., Jiang, L., & Liu, J. F. (2024). The 1000 Chinese Indigenous Pig Genomes Project provides insights into the genomic architecture of pigs. Nature Communications, 15(1), 10137. https://doi.org/10.1038/s41467-024-54471-z
  • 59. Boichard, D., Ducrocq, V., Croiseau, P., & Fritz, S. (2016). Genomic selection in domestic animals: Principles, applications and perspectives. Comptes Rendus Biologies, 339(7- 8), 274–277. https://doi.org/10.1016/j.crvi.2016.04.007