Свинарство 2025 №5-6(83-84)

β-GLUCANS AS NATURAL IMMUNOSTIMULANTS IN AQUACULTURE

УДК: УДК 639.3.04:57.083.3

DOI: 10.37143/2786-7730-2025-5-6(83-84)12

БІБЛІОГРАФІЯ за ДСТУ: Tkaczenko H., Kurhaluk N. β-glucans as natural immunostimulants in aquaculture. Свинарство і агропромислове виробництво : міжвідом. темат. наук. зб. / Ін-т свинарства і АПВ НААН. Полтава, 2025. Вип. 5–6(83–84). С. 184–194. 10.37143/2786-7730-2025-5-6(83-84)12

H. M. Tkaczenko, Doctor of Biological Sciences, Professor, Vice-Director of Institute of Biology
ORCID:https//orcid.org/0000-0003-3951-9005
E-mail:: halina.tkaczenko@upsl.edu.pl
Institute o f Biology, Pomeranian University in Slupsk, 22B Arciszewskiego St., Slupsk, Poland 76-200 ROR: https://ror.org/00h8nar58
N. M. Kurhaluk, Doctor of Biological Sciences, Professor
ORCID:https//orcid.org/0000-0002-4669-1092
E-mail:natalia.kurhaluk@upsl.edu.pl
Institute o f Biology, Pomeranian University in Slupsk, 22B Arciszewskiego St., Slupsk, Poland 76-200 ROR: https://ror.org/00h8nar58

Рукопис надійшов/ 15.10.2025

Після рецензування/30.10.2025

Прийнято до друку/ 15.11.2025

Доступно онлайн/ 30.12.2025

Анотація

Aquaculture is one of the fastest-growing sectors of global food production; however, the intensification of farming practices has resulted in increased disease prevalence, elevated stress levels in cultured fish and a growing dependence on antibiotics. These challenges highlight the urgent need for sustainable, environmentally safe and biologically effective alternatives for health management in aquaculture systems. The objective of this review was to analyse the immunostimulatory potential of β-glucans as natural feed additives in aquaculture, with particular emphasis on their mechanisms of action, physiological effects and practical applicability in intensive fish farming. Methods applied included a critical analysis and synthesis of experimental and review studies published in international peer-reviewed journals, focusing on molecular, cellular and organism-level responses of fish to β-glucan administration. Data concerning β-glucan structure, receptor-mediated recognition, immune signalling pathways and functional outcomes were systematically evaluated. Results demonstrate that β- glucans significantly enhance innate and adaptive immune responses in fish through activation of macrophages, neutrophils and other immune effector cells via pattern recognition receptors, including C-type lectins, complement- related receptors and Toll-like receptors. Their administration leads to increased phagocytic activity, cytokine production and improved resistance to bacterial, viral and parasitic infections. In addition to immune modulation, β-glucans positively influence growth performance, antioxidant capacity, gut health and stress resilience under intensive aquaculture conditions. Evidence also suggests the induction of long-term functional reprogramming of innate immune cells, known as trained immunity, which may contribute to prolonged non-specific protection in teleost fish. Conclusions indicate that β- glucans represent one of the most promising natural immunostimulants for sustainable and antibiotic-free aquaculture. Nevertheless, variability in β-glucan sources, molecular structures, dosages and delivery methods remains a critical limitation. Further standardisation and advanced transcriptomic, proteomic and metabolomic studies are required to optimise their application and fully elucidate immune–endocrine interactions in fish.

Keywords: β-glucans, aquaculture, fish immunity, immunostimulation, functional feeds, disease resistance, sustainable production

БІБЛІОГРАФІЯ

1. Doan, H. V., Sumon, M. A. A., Tran, H. Q., Le, C. X., Mohammady, E. Y., El-Haroun, E. R., Hoseinifar, S. H., Ringo, E., Stejskal, V., & Dawood, M. A. O. (2024). Role of β-glucan on finfish and shellfish health and well-being: A systematic review and meta-analysis. Reviews in Aquaculture, 16(4), 1996–2009. https://doi.org/10.1111/raq.12944
2. Verdegem, M., Buschmann, A. H., Latt, U. W., Dalsgaard, A. J. T., & Lovatelli, A. (2023). The contribution of aquaculture systems to global aquaculture production. J of the World Aquaculture Society, 54(2), 206–250. https://doi.org/10.1111/jwas.12963
3. Gonzalez, T. J. (2023). ‘Positive’ animal welfare in aquaculture as a cardinal principle for sustainable development. Frontiers in Animal Science, 4, 1206035. https://doi.org/10.3389/fanim.2023.1206035
4. Singh, B. K., Delgado-Baquerizo, M., Egidi, E., Guirado, E., Leach, J. E., Liu, H., & Trivedi, P. (2023). Climate change impacts on plant pathogens, food security and paths forward. Nature reviews. Microbiology, 21(10), 640–656. https://doi.org/10.1038/s41579-023-00900-7
5. Caputo, A., Bondad-Reantaso, M. G., Karunasagar, I., Hao, B., Gaunt, P., Verner- Jeffreys, D., Fridman, S., & Dorado-Garcia, A. (2023). Antimicrobial resistance in aquaculture: A global analysis of literature and national action plans. Reviews in Aquaculture, 15(2), 568–578. https://doi.org/10.1111/raq.12741
6. Vetvicka, V., Vannucci, L., & Sima, P. (2013). The Effects of β - Glucan on Fish Immunity. North American J of Medical Sci., 5(10), 580–588. https://doi.org/10.4103/1947-2714.120792
7. Dimler, R. J., Davis, H. A., & Hilbert, G. E. (1946). A new anhydride of d-glucose: d-glucosan <1,4> β <1,6>. J. American Chem. Society, 68(7), 1377–1380. https://doi.org/10.1021/ja01211a085
8. Wooles, W. R., & DiLuzio, N. R. (1963). Reticuloendothelial function and the immune response. Science, 142(3595), 1078–1080. https://doi.org/10.1126/science.142.3595.1078
9. Rodrigues, M. V., Zanuzzo, F. S., Koch, J. F. A., de Oliveira, C. A. F., Sima, P., & Vetvicka, V. (2020). Development of Fish Immunity and the Role of β-Glucan in Immune Responses. Molecules (Basel, Switzerland), 25(22), 5378. https://doi.org/10.3390/molecules25225378
10. Xia, Y., Vetvicka, V., Yan, J., Hanikýrová, M., Mayadas, T., & Ross, G. D. (1999). The beta-glucan-binding lectin site of mouse CR3 (CD11b/CD18) and its function in generating a primed state of the receptor that mediates cytotoxic activation in response to iC3b- opsonized target cells. J of Immunology (Baltimore, Md.: 1950), 162(4), 2281–2290.
11. Brown, G. D., Herre, J., Williams, D. L., Willment, J. A., Marshall, A. S., & Gordon, S. (2003). Dectin-1 mediates the biological effects of beta-glucans. The J of Experimental Med., 197(9), 1119–1124. https://doi.org/10.1084/jem.20021890
12. Elder, M. J., Webster, S. J., Chee, R., Williams, D. L., Hill Gaston, J. S., & Goodall, J. C. (2017). β-Glucan Size Controls Dectin-1-Mediated Immune Responses in Human Dendritic Cells by Regulating IL-1β Production. Frontiers in Immunology, 8, 791. https://doi.org/10.3389/fimmu.2017.00791
13. Goodridge, H. S., Wolf, A. J., & Underhill, D. M. (2009). Beta-glucan recognition by the innate immune system. Immunological Reviews, 230(1), 38–50. https://doi.org/10.1111/j.1600-065X.2009.00793.x
14. Jin, Y., Li, P., & Wang, F. (2018). β-glucans as potential immunoadjuvants: A review on the adjuvanticity, structure-activity relationship and receptor recognition properties. Vaccine, 36(35), 5235–5244. https://doi.org/10.1016/j.vaccine.2018.07.038
15. Brown, G. D., Taylor, P. R., Reid, D. M., Willment, J. A., Williams, D. L., Martinez- Pomares, L., Wong, S. Y., & Gordon, S. (2002). Dectin-1 is a major beta-glucan receptor on macrophages. The J of Experimental Med., 196(3), 407–412. https://doi.org/10.1084/jem.2002047
16. Cohen, N. R., Tatituri, R. V., Rivera, A., Watts, G. F., Kim, E. Y., Chiba, A., Fuchs, B. B., Mylonakis, E., Besra, G. S., Levitz, S. M., Brigl, M., & Brenner, M. B. (2011). Innate recognition of cell wall β-glucans drives invariant natural killer T cell responses against fungi. Cell Host & Microbe, 10(5), 437–450. https://doi.org/10.1016/j.chom.2011.09.011
17. Aizawa, M., Watanabe, K., Tominari, T., Matsumoto, C., Hirata, M., Grundler, F. M. W., Inada, M., & Miyaura, C. (2018). Low Molecular-Weight Curdlan, (1→3)-β-Glucan Suppresses TLR2-Induced RANKL-Dependent Bone Resorption. Biological & Pharmaceutical Bulletin, 41(8), 1282–1285. https://doi.org/10.1248/bpb.b18-00057
18. Goodridge, H. S., Reyes, C. N., Becker, C. A., Katsumoto, T. R., Ma, J., Wolf, A. J., Bose, N., Chan, A. S., Magee, A. S., Danielson, M. E., Weiss, A., Vasilakos, J. P., & Underhill, D. M. (2011). Activation of the innate immune receptor Dectin-1 upon formation of a 'phagocytic synapse'. Nature, 472(7344), 471–475. https://doi.org/10.1038/nature10071
19. Ferwerda, G., Meyer-Wentrup, F., Kullberg, B. J., Netea, M. G., & Adema, G. J. (2008). Dectin-1 synergizes with TLR2 and TLR4 for cytokine production in human primary monocytes and macrophages. Cellular Microbiology, 10(10), 2058–2066. https://doi.org/10.1111/j.1462-5822.2008.01188.x
20. Liu, M., Tong, Z., Ding, C., Luo, F., Wu, S., Wu, C., Albeituni, S., He, L., Hu, X., Tieri, D., Rouchka, E. C., Hamada, M., Takahashi, S., Gibb, A. A., Kloecker, G., Zhang, H. G., Bousamra, M., II, Hill, B. G., Zhang, X., & Yan, J. (2020). Transcription factor c-Maf is a checkpoint that programs macrophages in lung cancer. The J of Clinical Investigation, 130(4), 2081–2096. https://doi.org/10.1172/JCI131335
21. Magnadóttir B. (2006). Innate immunity of fish (overview). Fish & shellfish immunology, 20(2), 137–151. https://doi.org/10.1016/j.fsi.2004.09.006
22. Petit, J., Bailey, E. C., Wheeler, R. T., de Oliveira, C. A. F., Forlenza, M., & Wiegertjes, G. F. (2019). Studies Into β-Glucan Recognition in Fish Suggests a Key Role for the C-Type Lectin Pathway. Frontiers in Immunology, 10, 280. https://doi.org/10.3389/fimmu.2019.00280
23. Magnadottir B. (2010). Immunological control of fish diseases. Marine Biotechnology (New York, N.Y.), 12(4), 361–379. https://doi.org/10.1007/s10126-010-9279-x
24. Løvoll, M., Fischer, U., Mathisen, G. S., Bøgwald, J., Ototake, M., & Dalmo, R. A. (2007). The C3 subtypes are differentially regulated after immunostimulation in rainbow trout, but head kidney macrophages do not contribute to C3 transcription. Vet Immunology and Immunopathology, 117(3-4), 284–295. https://doi.org/10.1016/j.vetimm.2007.03.005
25. Chansue, N., Endo, M., Kono, T., & Sakai, M. (2000). The stimulation of cytokine-like protein in tilapia (Oreochromis niloticus) orally treated with β-1,3 glucan. Asian Fisheries Sci., 13, 271–278.
26. Hardie, L. J., Chappell, L. H., & Secombes, C. J. (1994). Human tumor necrosis factor alpha influences rainbow trout Oncorhynchus mykiss leucocyte responses. Vet Immunology and Immunopathology, 40(1), 73–84. https://doi.org/10.1016/0165-2427(94)90016-7
27. Kim, Y. S., Ke, F., & Zhang, Q. Y. (2009). Effect of beta-glucan on activity of antioxidant enzymes and Mx gene expression in virus infected grass carp. Fish & Shellfish Immunology, 27(2), 336–340. https://doi.org/10.1016/j.fsi.2009.06.006
28. Lokesh, J., Fernandes, J. M., Korsnes, K., Bergh, O., Brinchmann, M. F., & Kiron, V. (2012). Transcriptional regulation of cytokines in the intestine of Atlantic cod fed yeast derived mannan oligosaccharide or β-glucan and challenged with Vibrio Anguillarum. Fish & Shellfish Immunology, 33(3), 626–631. https://doi.org/10.1016/j.fsi.2012.06.017
29. Marel, M.v, Adamek, M., Gonzalez, S. F., Frost, P., Rombout, J. H., Wiegertjes, G. F., Savelkoul, H. F., & Steinhagen, D. (2012). Molecular cloning and expression of two β-defensin and two mucin genes in common carp (Cyprinus carpio L.) and their up-regulation after β- glucan feeding. Fish & Shellfish Immunology, 32(3), 494–501. https://doi.org/10.1016/j.fsi.2011.12.008
30. Falco, A., Frost, P., Miest, J., Pionnier, N., Irnazarow, I., & Hoole, D. (2012). Reduced inflammatory response to Aeromonas salmonicida infection in common carp (Cyprinus carpio L.) fed with β-glucan supplements. Fish & shellfish immunology, 32(6), 1051–1057. https://doi.org/10.1016/j.fsi.2012.02.028
31. Miest, J. J., Falco, A., Pionnier, N. P., Frost, P., Irnazarow, I., Williams, G. T., & Hoole, D. (2012). The influence of dietary β-glucan, PAMP exposure and Aeromonas salmonicida on apoptosis modulation in common carp (Cyprinus carpio). Fish & Shellfish Immunology, 33(4), 846–856. https://doi.org/10.1016/j.fsi.2012.07.014
32. Ghaedi, G., Keyvanshokooh, S., Mohammadi Azarm, H., & Akhlaghi, M. (2016). Proteomic analysis of muscle tissue from rainbow trout (Oncorhynchus mykiss) fed dietary β- glucan. Iranian J of Vet Research, 17(3), 184–189.
33. Dawood, M. A. O., Abdo, S. E., Gewaily, M. S., Moustafa, E. M., SaadAllah, M. S., AbdEl-Kader, M. F., Hamouda, A. H., Omar, A. A., & Alwakeel, R. A. (2020). The influence of dietary β-glucan on immune, transcriptomic, inflammatory and histopathology disorders caused by deltamethrin toxicity in Nile tilapia (Oreochromis niloticus). Fish & Shellfish Immunology, 98, 301–311. https://doi.org/10.1016/j.fsi.2020.01.035
34. Soltanian, S., Adloo, M. N., Hafeziyeh, M., & Ghadimi, N. (2014). Effect of β-glucan on cold-stress resistance of striped catfish, Pangasianodon Hypophthalmus (Sauvage, 1878). Vet Medicina, 59, 440–446. https://doi.org/10.17221/7684-VETMED
35. Divya, M., Gopi, N., Iswarya, A., Govindarajan, M., Alharbi, N. S., Kadaikunnan, S., Khaled, J. M., Almanaa, T. N., & Vaseeharan, B. (2020). β-glucan extracted from eukaryotic single-celled microorganism Saccharomyces cerevisiae: Dietary supplementation and enhanced ammonia stress tolerance on Oreochromis mossambicus. Microbial Pathogenesis, 139, 103917. https://doi.org/10.1016/j.micpath.2019.103917
36. Petit, J., & Wiegertjes, G. F. (2016). Long-lived effects of administering β-glucans: Indications for trained immunity in fish. Developmental and Comparative Immunology, 64, 93–102. https://doi.org/10.1016/j.dci.2016.03.003
37. Netea, M. G., Quintin, J., & van der Meer, J. W. (2011). Trained immunity: a memory for innate host defense. Cell Host & Microbe, 9(5), 355–361. https://doi.org/10.1016/j.chom.2011.04.006
38. Kleinnijenhuis, J., Quintin, J., Preijers, F., Benn, C. S., Joosten, L. A., Jacobs, C., van Loenhout, J., Xavier, R. J., Aaby, P., van der Meer, J. W., van Crevel, R., & Netea, M. G. (2014). Long-lasting effects of BCG vaccination on both heterologous Th1/Th17 responses and innate trained immunity. J of Innate Immunity, 6(2), 152–158. https://doi.org/10.1159/000355628
39. Conrath U. (2006). Systemic acquired resistance. Plant Signaling & Behavior, 1(4), 179–184. https://doi.org/10.4161/psb.1.4.3221
40. Kurtz J. (2005). Specific memory within innate immune systems. Trends in immunology, 26(4), 186–192. https://doi.org/10.1016/j.it.2005.02.001
41. Olivier, G., Eaton, C. A., & Campbell, N. (1986). Interaction between Aeromonas salmonicida and peritoneal macrophages of brook trout (Salvelinus fontinalis). Vet Immunology and Immunopathology, 12(1-4), 223–234. https://doi.org/10.1016/0165-2427(86)90126-1
42. Kato, G., Kondo, H., Aoki, T., & Hirono, I. (2010). BCG vaccine confers adaptive immunity against Mycobacterium sp. infection in fish. Developmental and Comparative Immunology, 34(2), 133–140. https://doi.org/10.1016/j.dci.2009.08.013
43. Hohn, C., & Petrie-Hanson, L. (2012). Rag1-/- mutant zebrafish demonstrate specific protection following bacterial re-exposure. PloS One, 7(9), e44451. https://doi.org/10.1371/journal.pone.0044451
44. Meena, D. K., Das, P., Kumar, S., Mandal, S. C., Prusty, A. K., Singh, S. K., Akhtar, M. S., Behera, B. K., Kumar, K., Pal, A. K., & Mukherjee, S. C. (2013). Beta-glucan: an ideal immunostimulant in aquaculture (a review). Fish Physiology and Biochemistry, 39(3), 431–457. https://doi.org/10.1007/s10695-012-9710-5
45. Selvaraj, V., Sampath, K., & Sekar, V. (2005). Administration of yeast glucan enhances survival and some non-specific and specific immune parameters in carp (Cyprinus carpio) infected with Aeromonas hydrophila. Fish & Shellfish Immunology, 19(4), 293–306. https://doi.org/10.1016/j.fsi.2005.01.001
46. Jorgensen, J. B., Sharp, G. J., Secombes, C. J., & Robertsen, B. (1993). Effects of a yeast-cell-wall glucan on the bactericidal activity of rainbow trout macrophages. Fish & Shellfish Immunology, 3, 267–277.
47. Sveinbjornsson, B., Smedsrød, B., Berg, T., & Seljelid, R. (1995). Intestinal uptake and organ distribution of immunomodulatory aminated β-1,3-D-polyglucose in Atlantic salmon (Salmo salar L.). Fish & Shellfish Immunology, 5, 39–50.
48. Robertsen, B., Rørstad, G., Engstad, R., & Raa, J. (1990). Enhancement of non- specific resistance in Atlantic salmon, Salmo salar L., by a glucan from Saccharomyces cerevisiae cell walls. J of Fish Diseases, 13, 391–400.
49. Nikl, L., Albright, L. J., & Evelyn, T. P. (1991). Influence of seven immunostimulants on the immune response of coho salmon to Aeromonas Salmonicida. Diseases of Aquatic Organisms, 12, 7–12.
50. Midtlyng, P. J., Reitan, L. J., & Speilberg, L. (1996). Experimental studies on the efficacy and side-effects of intraperitoneal vaccination of Atlantic salmon (Salmo salar L.) against furunculosis. Fish & Shellfish Immunology, 6, 335–350.
51. Verlhac, V., Gabaudan, J., Obach, A., Schuep, W., & Hole, R. (1996). Influence of dietary glucan and vitamin C on non-specific and specific immune responses of rainbow trout (Oncorhynchus mykiss). Aquaculture, 143, 123–133.