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THE INTERDEPARTMENTAL SUBJECT SCIENTIFIC DIGEST PIG BREEDING

and agroindustrial production

INTRAHEPATIC BILE DUCTES OF HUMANS AND PIGS: STRUCTURAL ADAPTATIONS TO THE DIET COMPOSITION


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UDC: 591.436:[636.4+611.36]

DOI: 10.37143/2786-7730-2024-4(82)1

REFERENCIS АРА style:Dubinin, D. S., Zinoviev, S. G., & Saienko A. M. (2024). Vnutrishnopechinkovi zhovchni protoky liudyny ta svyni: strukturni adaptatsii do skladu ratsionu. [Intrahepatic bile ductes of humans and pigs: structural adaptations to the diet composition]. Svynarstvo i ahropromyslove vyrobnytstvo [Pig Breeding and Agroindustrial Production]. Poltava, 4(82), 7-20 [in Ukrainіаn]. doi: 10.37143/2786-7730-2024-4(82)1

D. S. Dubinin, Doctor of Philosophy, Research Fellow, Laboratory of Animal Nutrition, Physiology and Health,
ORCID:https://orcid.org/0000-0002-5547-1614
E-mail:dmytrudubinin4@gmai.com
2Institute o f Pig Breeding and Agro-Industrial Production o f the NAAS 1 Swedish Mogyla, Poltava, Ukraine, 36013
S. G. Zinoviev, candidate of agricultural sciences, senior researcher, acting head of the lab. environmental safety in animal husbandry
ORCID:https://orcid.org/0000-0002-3757-3860
E-mail:kvazimodo2077@gmail.com
Institute of Pig Breeding and agroindustrial production NAAS, Shvedska Mohyla Str., 1, Poltava, Ukraine, 36009
A. M. Saienko, Candidate of Agricultural Sciences, Senior Researcher, Acting Head of the Genetic Laboratory,
ORCID:https://orcid.org/0000-0002-0527-5367
E-mail:saenko_artem@meta.ua
Institute of Pig Breeding and agroindustrial production NAAS, Shvedska Mohyla Str., 1, Poltava, Ukraine, 36009

Abstract

Objective.The purpose o f the work was to study the structural organization o f the intrahepatic biliary tract in humans and pigs in a comparative anatomical aspect to determine common and distinctive features caused by the structure o f the diet, nutritional characteristics, and functional load. Methods. In the study it was used the general histological methods to analyze the structure o f the walls o f the intrahepatic bile ducts. The preparations were stained with hematoxylin and eosin. For proper fixation, the samples were washed with 10 % neutral formalin solution, after which the macropreparations were immersed in the same solution for further fixation. 15 samples o f liver from pigs with a live weight o f 90-105 kg, housed in the conditions o f the farm with dry-type feeding, were studied. A similar study was conducted on 15 liver samples from people aged 30 to 50 years who died without any detected pathologies o f the hepatobiliary system. Organ removal was carried out no later than 12 hours after death. The structural organization o f the walls o f the intrahepatic bile ducts was analyzed. Results. The structure o f the pig interlobular bile duct is similar to that o f the human, but has certain morphometric differences due to the peculiarities o f their diet and nutrition structure. Cubical epithelial cells predominate in the mucosa, although their number is somewhat higher - about 18.04±1.73 cells on a cross-section. Analysis o f the structure o f human intersegmental bile ducts showed that the mucous membrane is represented mainly by cubic epithelium, although prismatic cells are also found. The average number o f epithelial cells on a cross-section was about 20.21±2.03 cells. In pigs, intersegmental bile ducts have certain differences compared to humans. The inner epithelial layer is represented by prismatic cells, which are larger in size. The total number o f epithelial cells in the cross section rangedfrom 24.54±2.03 cells. The study showed that the human interlobular bile ducts have a two-layer structure: the inner layer is formed by prismatic epithelium, and the outer layer is loose connective tissue. Epitheliocytes were located on the basement membrane in a single layer, their average number in the cross section was 28.65±5.03 cells. The interlobular bile ducts o f the pig had a similar morphological organization, but certain features were observed. Epitheliocytes had a prismatic shape and were somewhat smaller in size than in humans. The average number o f cells in the cross section was 36.45±4.73 cells. Conclusions: No significant differences in the structural organization o f the intrahepatic bile ducts o f humans and pigs were found. Minor differences in morphometric parameters may be associated with the characteristics o f the body size, but the overall histoarchitectonics remains similar.

Keywords: liver, biliary tract, cholecystitis, cholelithiasis, omnivorous mammals, bile ducts, pig, histology, epitheliocytes

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