Pig Breeding 2024 №3(81)

BAKTERITSIDNA AKTIVNIST PREPARATIV NA OSNOVI JUGLANS REGIA PO VIDNOSHENNYU DO PASPORTIZOVANIH BAKTERIALNIH TEST-KULTUR

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UDC: 615.322:615.281.9

DOI: 10.37143/2786-7730-2024-3(81)11

REFERENCIS АРА style:Hrubich. P. Yu., Kurman. A. F.. Pushkina. M. L. Sinitsyn, O. S. (2024). Bactericidal activity of Juglans regia - based preparations against certified bacterial test cultures [ Bactericidal activity of preparations based on Juglans Regia against certified bacterial test cultures]. Svynarstvo і ahropromvslove vyrobnytstvo [Pig Breeding and Agroindustrial Production], Poltava. 3(81). 155-167 [inUkrainain], doi: 10.37143/2786-7730-2024-3(81)11

P. Yu. Hrubich,Candidate of Veterinary Sciences, Senior Researcher, Head of the Bacteriological Laboratory of LLC "Vetsintez JSC"
ORCID:ORCID 0000-0002-9622-2268
E-mail:gruba@i.ua
TOV ”JSC Vetsintez”56 Chuguivska St., Kharkiv, Ukraine, 61140
A. F. Kurman candidate of biological sciences Associate Professor, Senior Research Fellow, Laboratory of Animal Nutrition, Physiology and Health,
ORCID:ORCID 0000-0002-7188-2659
E-mail:andikurman@ukr.net
Institute of Pig Breeding and agroindustrial production NAAS, Shvedska Mohyla Str., 1, Poltava, Ukraine, 36013
M. L. Pushkina junior researcher, animal nutrition, physiology and health laboratories
ORCID:https://orcid.org/0000-0001-5705-2977
E-mail:azulaniakris@gmail.com
Institute of Pig Breeding and agroindustrial production NAAS, Shvedska Mohyla Str., 1, Poltava, Ukraine, 36013
Sinitsyn O. Spostgraduate
ORCID:https://orcid.org/0009-0006-5015-902X
E-mail:alex.senicen@gmail.com
2Institute o f Pig Breeding and Agro-Industrial Production o f the NAAS 1 Swedish Mogyla, Poltava, Ukraine, 36013

Abstract

Objective. To study the bactericidal activity o f a number ofpreparations obtained by different technological processes from plant materials o f individual parts o f the Green walnuts on several certified model strains o f bacteria that differ in biological diversity. To determine the disinfectant capabilities o f these preparations in the absence o f antagonistic and synergistic effects o f the fu ll range o f mixtures o f typical banal microflora o f pig farms. Methods. The following methods were used: biological and botanical to determine the phase o f vegetation o f Juglans regia in the selection o f raw materials from different parts o f the plant; biochemical and chemical in the process o f preparation and isolation o f the active substance from these samples and their purification from ballast fiber by maceration and extraction, using a number o f devices and equipment; microbiological fo r the preparation o f standardized culture media with selective properties that correspond to the peculiarities o f reproduction, development and distribution o f selected model strains. Surface inoculation was performed on liquid and agar-based culture media in test tubes and Petri dishes using the standard streak plate method with a microbiological loop. Subsequently, standard incubation at 37°C for 24 hours was performed. Results. It was found that the greatest bactericidal and fungicidal activity, and therefore potential effectiveness as a possible disinfectant in organic pig production technology, was found in extracts o f phytomaterials o f Juglans regia L - potential disinfectants No. 2 (extractant - 5 % acetic acid, wax-ripening nut) and No. 4 (extractant - 20 % alcohol, wax ripening nut) - brought to 5 % alcohol after obtaining the primary extract. Conclusions. According to the results o f studies on certified strains o f microorganisms, it was found that the studied extracts have a significant effect on representatives o f enterobacteria, a weak effect on spore forming bacilli

Key words: organic pig production, bactericidal activity, phytodesinfectant, certified test cultures, Salmonella typhimurium, Bacillus subtilis, extract, walnut.

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