DOI: 10.37143/0371-4365-2021-75-76-05

Y. O. Budakva, д. с.-г. н., аспірантка, лаб. генетики,
ORCID:https://orcid.org/0000-0001-5941-1953
E-mail:budakvayelyzaveta@gmail.com
Institute of Pig Breeding and agroindustrial production NAAS, Shvedska Mohyla Str., 1, Poltava, Ukraine, 36013
K. F. Pochernyaev, д. с.-г. н., заст. директора з науково-виробничої і фінансової діяльності
ORCID:https://orcid.org/0000-0001-9973-6429
E-mail:k.f.pochernyaev@gmail.com
Institute of Pig Breeding and agroindustrial production NAAS, Shvedska Mohyla Str., 1, Poltava, Ukraine, 36013
S. M Korinnyiк. с.-г. наук, с. н. с., доцент кафедри біотехнології та хімії,
ORCID:https://orcid.org/0000-0002-1649-3079
E-mail:korinny_sergey@ukr.net
Poltava State Agrarian University Skovoroda Str., 1/3, Poltava, Ukraine, 36003
M. G. Povodдоктор с.-г. наук, професор кафедри технології кормів і годівлі тварин,
ORCID:https://orcid.org:0000-0001-9272-9672
E-mail:nic.pov@ukr.net
Sumy National Agrarian University Herasyma Kondratieva Str., 160, Sumy, Ukraine, 40000

Abstract

The purpose of this work was to determine the origin of pigs of the final hybrid (Large White×Landrace)×Maxgro using mitochondrial DNA markers. Bristle from the auricle of pigs (Large White×Landrace)×Maxgro was used as genetic material. DNA isolation was carried out according to the method of S. M. Korinnyi et al. 2005, using the Chelex - 100 ion exchange resin. The method of PCR-amplified restriction fragment length polymorphism (PCR-RFLP) was used to analyze the mitochondrial genome. Sections of the D-loop of the pig mitochondrial genome with a size of 428 nucleotide pairs (with Tas 1 recognition sites at positions 15558, 15580, 15616, 15714, 15758) are subject to analysis. The comparison of maternally inherited restriction fragments in the breed made it possible to obtain reliable information about the origin of the experimentally studied sample of pigs of the final hybrid (n=15) from LLC "Globynsky Svynocompleks", Globyno, Poltava Region, Ukraine.The laboratory research was carried out on the basis of the Institute of Pig Breeding and AIP of the National Academy of Agrarian Sciences of Ukraine in the laboratory of genetics. By analyzing nucleotide sequences, one monomorphic site at position 15558 bp and three polymorphic sites at positions 15580, 15616, and 15758 were identified and experimentally studied for endonuclease Tas I (↓AATT). To systematize the combinations of restriction fragments of mitochondrial DNA, three breed-specific mitochondrial haplotypes were determined in the experimentally studied sample of hybrid pigs (n=15). As a result it was obtained the haplotypes of the studied pig population (Large White×Landrace)×Maxgro according to mitochondrial DNA markers: 4 animals with haplotype C - Landrace, Hampshire, Wales,a subspecies of wild pig (Ukraine, Poland); 5 pigs have mitochondrial haplotype N –the Large White (Asian type), Berkshire, the Asian wild boar; 6 pigs with mitochondrial haplotype O are Landraces, a subspecies of wild boar (Scandinavia). The obtained results indicate that two-breed sows were the result of direct the (Large White×Landrace) and reciprocal crossing (Landrace×Large White). Based on the example of the developed systematization of the combination of restriction fragments by Pocherniaev K. F., in the future it is proposed to create a database of reference haplotypes of mitochondrial DNA of pigs of the final hybrid (Large White×Landrace)×Maxgro. It will be used in future studies to reconstruct the demographic history of commercial breeds of hybrid pigs of foreign selection. The work was carried out with the support of the National Academy of Agrarian Sciences of Ukraine 31.01.00.07.F. "To investigate the pleiotropic effect of genes whose SNPs are used in marker-associated selection of pigs." SR No. 0121U109838.

Key words: mitochondrial DNA, D-loop, pigs, final hybrid, (Large White×Landrace)×Maxgro, haplotype, haplogroup, PCR-PDRF, selection, origin.

REFERENCES

• 1. Fumihito, A, Miyake, T, Sumi, S, Takada, M, Ohno, S, & Kondo, N. (1995). One subspecies of the red junglefowl (Gallus gallus gallus) suffices as the matriarchic ancestor of all domestic breeds. Proceedings of the National Academy of Sciences of the United States of America, 91(26), 12505–12509. doi: 10.1073/pnas.91.26.12505
• 2. Niu, T., Qin, Z. S., Xu, X., & Liu, J. S. (2002). Bayesian Haplotype Inference for Multiple Linked Single-Nucleotide Polymorphisms. American Journal of Human Genetics 70(1), 157–169. doi: 10.1086%2F338446
• 3. Liu, H., Wang, J., Wang, D., Kong, M., Ning, C., Zhang, X., Xiao, J., Zhang, X., Liu, J., & Zhao, X. (2020). Cybrid Model Supports Mitochondrial Genetic Effect on Pig Litter Size. Frontiers in Genetics, 11, 1–10. doi: https://doi.org/10.3389/fgene.2020.579382
• 4. Kolosova, M. A., Bakoev, N. F., Kolosov, A. Yu., & Getmantseva, L. V., (2029). Izuchenie gena SOH2 mtDNK svinej razlichnogo proishozhdenija [Study of mitochondrial COX2 DNA gene of pigs of different origin]. Mezhdunarodnyj nauchno- issledovatel'skij zhurnal. [International research journal]. Ekatirenburg, 1(79), 2, 10–13. doi: 10.23670/IRJ.2019.79.1.030 [in Russian].
• 5. Te-Sha Tsai, Sriram Rajasekar, Justin C. St. John. (2016). The relationship between mitochondrial DNA haplotype and the reproductive capacity of domestic pigs (Sus scrofa domesticus). BMC Genetics. 17(67). 1–17. doi: 10.1186/s12863-016-0375-4.
• 6. Yeung, Ka Yu., Dickinson, A., Donoghue, J. F., Polekhina, G., White, S. J., Grammatopoulos, D. K., McKenzie, M., Johns, T. G., & John, J. C. St. (2014). The identification of mitochondrial DNA variants in glioblastoma multiforme. Acta Neuropathologica Communications, 2(1), 2–21. doi: 10.1186/2051-5960-2-1.
• 7. Yu, G., Xiang, H., Wang. J., & Zhao, X. (2013). The phylogenetic status of typical Chinese native pigs: analyzed by Asian and European pig mitochondrial genome sequences. Journal of Animal Science and Biotechnology, 4(9). doi: 10.1186/2049-1891-4-9
• 8. Origin and history of livestock diversity In Scherf, B. D., Pilling; D. (ed.) (2015). The second report on the state of the world’s Animal Genetic Resources for food and agriculture. Rome: FAO Commission on Genetic Resources for Food and Agriculture Assessments, 1 Section A, 5–16. Retrived from https://www.fao.org/3/i4787e/i4787e.pdf
• 9. Getmantseva, L., Bakoev, S., Bakoev, N., Karpushkina, T., & Kostyunina, O. (2020). Mitochondrial DNA Diversity in Large White Pigs in Russia. Animals, 10(8), Special Is. “Animal Genetics and Livestock Production: The Biodiversity Challenge”, 1365. doi: 10.3390/ani10081365
• 10. Korinnyi, S. M., Pochernyaev, K. F., & Balatsky, V. M. (2005). Sherstʹ tvaryn yak zruchnyi obiekt vydilennia DNK dlia analizu za dopomohoiu PLR [Animal fur as a convenient object of DNA isolation for PCR analysis]. Veterinary biotechnology, 7, 80–83 [in Ukrainian].
• 11. Pochernyaev K. F., Berezovsky M. D. (2014). The use of mitochondrial DNA markers to control the authenticity of origin of genealogical structures of sows. Methodical recommendations. 2014; 24–27. [in Ukrainian].
• 12. Lomako, D. M., Pochernyaev, K. F., & Bliznyuchenko, A. G. (2006). Markyrovanye semeystva svynomatok po haplotypam mytokhondryalʹnoy DNK [Marking of a family of sows by mitochondrial DNA haplotypes]. Bulletin of Agrarian Science of the Black Sea Region, 3(2), 71–74. Retrieved from http://visnyk.mnau.edu.ua/ [in Russian].