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S. M Korinnyiк. с.-г. наук, с. н. с., доцент кафедри біотехнології та хімії,
ORCID:https://orcid.org/0000-0002-1649-3079
E-mail:korinny_sergey@ukr.net
Poltava State Agrarian University Skovoroda Str., 1/3, Poltava, Ukraine, 36003
A. O. Onyshchenko, к. с.-г. н., с.н.с, в.о. зав. лаб. екологічної безпеки у тваринництві,
ORCID:https://orcid.org/0000-0002-0684-1201
E-mail:geroi76@ukr.net
Institute of Pig Breeding and agroindustrial production NAAS, Shvedska Mohyla Str., 1, Poltava, Ukraine, 36013
L. G. Peretiatko, к. с.-г. н., ст. н. с, зав. лаб. селекції,
ORCID:https://orcid.org/0000-0003-0919-8482
E-mail:lidipllper@ukr.net
Institute of Pig Breeding and agroindustrial production NAAS, Shvedska Mohyla Str., 1, Poltava, Ukraine, 36013
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Abstract
The aim of the research was genotyping animals of Ukrainian pig breeds according
to microsatellite loci of the genome for further creation and implementation of the
methodology for determining gene complexes of pig adaptability. Research methods:
zootechnical – the analysis of genealogy and the productivity indexes of animals of
different breeds; bioinformation - work with databases of the primary genome sequences
for the design of oligonucleotide primers; molecular genetic - nucleic acid extraction, the
determination of purity and concentration of DNA preparations, the amplification of
microsatellite loci in PCR with subsequent electrophoretic separation of the amplification
products in denaturing gel; statistical – the determination of population-genetic
parameters on the basis of typing data on microsatellite loci, the relationship of
microsatellite loci with performance indexes, the search for adaptive complexes. It was
carried out genotyping animals of the Ukrainian meat breed in SE “RF Elita MIW named
after V. M. Remeslo NAAS "of Kyiv region for 6 microsatellite loci with tetranucleotide
repeats, a panel of primers of its own design. It was found the fact that the average
number of alleles per locus was 5.2 alleles. The level of actual heterozygosity was 0.830,
while the expected heterozygosity was 0.780. However, this difference is not significant.
DNA typing animals for 6 loci of microsatellite DNA of 17 heads of Poltava meat breed
of SE "EB Nadiia" was carried out. As a result of genotyping, the average number of
alleles per locus was 3.8. The actual and expected heterozygosity levels were 0.680 and
0.730, respectively. By loci of microsatellite DNA, we found in animals of Myrhorod
breed: for locus FH3628 – 5 alleles of 200-228 nucleotides, FH1865 – 5 alleles (size from
109 to 129 nucleotides), FH4219 – 2 alleles of 80 and 92 nucleotides, FH1885 – 4 alleles
from 205 up to 213 nucleotides, FH3764 – 2 alleles 138 and 146 nucleotides, and for
FH4231 – 2 alleles 116 and 120 nucleotides. The level of actual heterozygosity was 0.860,
while the expected heterozygosity was 0.810. Thus, in three breeds of Ukrainian selection
for 6 loci of microsatellite DNA with tetranucleotide repeat, the level of actual
heterozygosity was in the range of 0.680 in animals of Poltava meat breed to 0.860 in
animals of Myrhorod breed. The level of expected heterozygosity is 0.730 in animals of
Poltava meat breed to 0.810 in Myrhorod breed. The high level of heterozygosity, as well
as the average number of alleles per locus allows to search for alleles and genotypes
associated with the adaptability of animals in breeds of pigs of Ukrainian selection.
Key words: pigs, microsatellite loci, genome, average number of alleles, level of
heterozygosity, population, variability
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