DOI: 10.37143/0371-4365-2020-74-11

S. V. Lobchenko, Researcher
ORCID:https://orcid.org/0000-0001-9469-6202
E-mail:svitlife@ukr.net
Institute of Pig Breeding and agroindustrial production NAAS, Shvedska Mohyla Str., 1, Poltava, Ukraine, 36013
T. O. Husar Junior Research Fellow
ORCID:
E-mail:pigbreeding@ukr.net
Institute of Pig Breeding and agroindustrial production NAAS, Shvedska Mohyla Str., 1, Poltava, Ukraine, 36013
B.O. Birta candidate of biological sciencesк
ORCID:
E-mail:pigbreeding@ukr.net
Institute of Pig Breeding and agroindustrial production NAAS, Shvedska Mohyla Str., 1, Poltava, Ukraine, 36013

Abstract

The results of studies of the viability of spermatozoa with different incubation time at different concentrations and using different diluents are highlighted in the article. (Un) concentrated spermatozoa were diluented: 1) with their native plasma; 2) medium 199; 3) a mixture of equal volumes of plasma and medium 199. The experiment was designed to generate experimental samples with spermatozoa concentrations prepared according to the method, namely: 0.2; 0.1; 0.05; 0.025 billion / ml. The sperm was evaluated after 2, 4, 6 and 8 hours. The perspective of such a study is significant and makes it possible to research various aspects of the subject in a wide range. In this regard, a series of experiments were conducted in this area. The data obtained are statistically processed and allow us to highlight the results that relate to each stage of the study. In particular, in this article it was found out some regularities between the viability of sperm, the type of diluent and the rate of rarefaction, as evidenced by the data presented in the tables. As a result of sperm incubation, the viability of spermatozoa remains at least the highest trend when sperm are diluted to a concentration of 0.1 billion / ml, regardless of the type of diluent used. To maintain the viability of sperm using this concentration of medium 199 is not better than its native plasma, and its mixture with an equal volume of plasma through any length of time incubation of such sperm. Most often it is at this concentration of sperm that their viability is characterized by the lowest coefficient of variation, regardless of the type of diluent used, which may indicate the greatest stability of the result under these conditions. The viability of spermatozoa with a concentration of 0.1 billion / ml is statistically significantly reduced only after 6 or even 8 hours of incubation. If the sperm are incubated for only 2 hours, regardless of the type of diluent used, the sperm concentrations tested do not affect the viability of the sperm

Key words: boar, spermatozoa, sperm plasma, concentration, incubation, medium 199, activity, viability, rarefaction.

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